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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
19/02/2016 |
Data da última atualização: |
26/06/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GERN, J. C.; SANTOS, L. F. dos; HABU, S.; NOSEDA, M. D.; LIMA, L. F. O. de; SILVA, A. L. L. da; SOCCOL, V. T.; BRANDAO, H. de M.; SOCCOL, C. R. |
Afiliação: |
JULIANA CARINE GERN, CNPGL; LEANDRO FREIRE dos SANTOS, Universidade Federal do Paraná; SASCHA HABU, Universidade Federal do Paraná; USP; MIGUEL DANIEL NOSEDA, Universidade Federal do Paraná; LUCIANNA FREITAS OLIVEIRA de LIMA, Universidade Federal do Paraná; ANDRÉ LUÍS LOPES da SILVA, Universidade Federal do Paraná; VANETE THOMAZ SOCCOL, Universidade Federal do Paraná; HUMBERTO DE MELLO BRANDAO, CNPGL; CARLOS RICARDO SOCCOL, Universidade Federal do Paraná. |
Título: |
Exopolysaccharide from Agaricus brasiliensis LPB and its Scale Up Studies in a Stirred Tank Fermenter. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Romanian Biotechnological Letters, v. 20, n. 6, p. 10945-10950, 2015. |
Idioma: |
Inglês |
Conteúdo: |
Background and purpose: Although the activities of polysaccharides such as beta-glucans are well known in Agaricus brasiliensis, there has been little research on their exopolysaccharides and its characterization or scale up studies. The aim of the study was to study the monosaccharide composition of the exopolysaccharide (EPS) produced by Agaricus brasiliensis LPB3 and its production in a stirred tank fermenter. Materials and methods: Agaricus brasiliensis LPB3 was cultured in submerged fermentation in a medium containing glucose, yeast extract, K2HPO4 and MgSO4. Extracellular polysaccharide was precipitated with four volumes of 95% ethanol. For monosaccharide composition, the EPSs were hydrolyzed, derivatized to alditol acetates, and analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). Results and conclusions: The exopolysaccharide produced by Agaricus brasiliensis was predominantly composed by mannose (57,7%) and galactose (28,2%) residues. The studies in a stirred tank fermenter kept the productivity obtained in laboratory scale regarding the production of exopolysaccharides (≈ 1200 mg/L). This result can be considered promising since scale up approach can decrease its yield. |
Palavras-Chave: |
Agaricus brasiliensis; Basidiomycete; Exopolysaccharide; Monosaccharide composition; Scale up; Stirred tank fermenter. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139533/1/Cnpgl-2015-RomBioLet-Exopolysaccharide.pdf
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Marc: |
LEADER 02098naa a2200289 a 4500 001 2037830 005 2023-06-26 008 2015 bl uuuu u00u1 u #d 100 1 $aGERN, J. C. 245 $aExopolysaccharide from Agaricus brasiliensis LPB and its Scale Up Studies in a Stirred Tank Fermenter.$h[electronic resource] 260 $c2015 520 $aBackground and purpose: Although the activities of polysaccharides such as beta-glucans are well known in Agaricus brasiliensis, there has been little research on their exopolysaccharides and its characterization or scale up studies. The aim of the study was to study the monosaccharide composition of the exopolysaccharide (EPS) produced by Agaricus brasiliensis LPB3 and its production in a stirred tank fermenter. Materials and methods: Agaricus brasiliensis LPB3 was cultured in submerged fermentation in a medium containing glucose, yeast extract, K2HPO4 and MgSO4. Extracellular polysaccharide was precipitated with four volumes of 95% ethanol. For monosaccharide composition, the EPSs were hydrolyzed, derivatized to alditol acetates, and analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). Results and conclusions: The exopolysaccharide produced by Agaricus brasiliensis was predominantly composed by mannose (57,7%) and galactose (28,2%) residues. The studies in a stirred tank fermenter kept the productivity obtained in laboratory scale regarding the production of exopolysaccharides (≈ 1200 mg/L). This result can be considered promising since scale up approach can decrease its yield. 653 $aAgaricus brasiliensis 653 $aBasidiomycete 653 $aExopolysaccharide 653 $aMonosaccharide composition 653 $aScale up 653 $aStirred tank fermenter 700 1 $aSANTOS, L. F. dos 700 1 $aHABU, S. 700 1 $aNOSEDA, M. D. 700 1 $aLIMA, L. F. O. de 700 1 $aSILVA, A. L. L. da 700 1 $aSOCCOL, V. T. 700 1 $aBRANDAO, H. de M. 700 1 $aSOCCOL, C. R. 773 $tRomanian Biotechnological Letters$gv. 20, n. 6, p. 10945-10950, 2015.
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Embrapa Gado de Leite (CNPGL) |
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Registros recuperados : 6 | |
1. | | BRONDANI, G. E.; WENDLING, I.; BRONDANI, A. E.; ARAUJO, M. A. de; SILVA, A. L. L. da; GONÇALVES, A. N. Dynamics of adventitious rooting in mini-cuttings of Eucalyptus benthamii x Eucalyptus dunnii. Acta Scientiarum. Agronomy, Maringá, v. 34, n. 2, p. 169-178, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Florestas. |
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2. | | HABIBI, P.; GROSSI DE SA, M. F.; MAKHZOUM, A.; MALIK, S.; SILVA, A. L. L. da; HEFFERON, K.; SOCCOL, C. R. Bioengineering hairy roots: phytoremediation, secondary metabolism, molecular pharming, plant-plant interactions and biofuels. In. LICHTFOUSE, E. (Ed.). Sustainable agriculture reviews. Springer, 2017. (Sustainable Agriculture Reviews, 22)Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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3. | | BRONDANI, G. E.; ARAUJO, M. A. de A.; AZEVEDO, J. H.; DUTRA, L. F.; SILVA, A. L. L. da; FIGUEIREDO, C. R. F. Intensidade luminosa e sacarose no cultivo in vitro de e.benthamii (Maiden & Cambage) X. E. dunni (Maiden) (Angiospermae:Myrtaceae). In: CONGRESSO NACIONAL DE BOTÂNICA, 60.; REUNIÃO NORDESTINA DE BOTÂNICA, 32.; ENCONTRO REGIONAL DE BOTÂNICOS - MG, BA , ES, 29., 2009, Feira de Santana. Botânica brasileira: futuro e compromissos. Feira de Santana: SBB: UEFS, 2009. 1 CD-ROM.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Clima Temperado. |
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4. | | HABIBI, P.; SA, M. F. G. de; SILVA, A. L. L. da; MAKHZOUM, A.; COSTA, J. da L.; BORGHETTI, I. A.; SOCCOL, C. R. Efficient genetic transformation and regeneration system from hairy root of Origanum vulgare. Physiology and Molecular Biology of Plants, v. 22, n. 2, p.271-277, 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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5. | | BRONDANI, G. E.; WENDLING, I.; PASCOA, M. A. R. de; OLIVEIRA, T. A. de; SILVA, A. L. L. da; ALMEIDA, M. de. Dinâmica de enraizamento de miniestacas de Eucalyptus benthamii x Eucalyptus dunnii. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para a produção de alimentos e bioenergia: livro de resumos. Fortaleza: SBFV: UFC: Embrapa Agroindústria Tropical, 2009. p. 32 Resumo.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Florestas. |
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6. | | GERN, J. C.; SANTOS, L. F. dos; HABU, S.; NOSEDA, M. D.; LIMA, L. F. O. de; SILVA, A. L. L. da; SOCCOL, V. T.; BRANDAO, H. de M.; SOCCOL, C. R. Exopolysaccharide from Agaricus brasiliensis LPB and its Scale Up Studies in a Stirred Tank Fermenter. Romanian Biotechnological Letters, v. 20, n. 6, p. 10945-10950, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Gado de Leite. |
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Registros recuperados : 6 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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